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ELECTROCHEMICAL IMMUNOSENSOR USING CdSe QUANTUM DOTS FOR ESTRADIOL DETECTION BASED ON ELECTRODEPOSITED GOLD NANOPARTICLES
Last modified: 2014-10-20
Abstract
17beta-Estradiol (E2), an endocrine disruptor compound (EDCs) endogenously produced by humans and mammals, has been released into the environment through the food chain. This might exert adverse effects on aquatic organisms by binding to the estrogen receptor, potentially affecting immunity and reproduction. The development of highly sensitive and specific analytical tools for determining E2 levels in environmental samples has become increasingly important for environmental monitoring. Electrochemical immunosensor detection has received increased attention in recent years due to the advantage of being highly sensitive, rapid, and inexpensive. It is also easy to measure the changes in electrical or electrochemical properties resulting from the antigen-antibody reactions on the surface of the sensor. Here, we attempted to develop an approach using electrodeposition of AuNPs onto the surface of the gold (Au) electrodes for signal amplification and to increase the total active surface area. The electrochemical properties of electrochemical sensors were characterized. The amount of E2 was determined by indirect determination of the amount of Cd2+ ions through differential pulse anodic stripping voltammetry (DPASV). The sensitivity of the resulting sensor for E2 detection was evaluated and compared between the non-coated Au electrode and AuNPs/Au electrode.
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